OSR1 CRISPR Activation Plasmid (h): sc-406034-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OSR1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- OSR1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by OSR1 CRISPR Activation Plasmid (h) and OSR1 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the OSR1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OSR1 Antibody (C-8): sc-376545
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OSR1 CRISPR Activation Plasmid (h) | sc-406034-ACT | 20 µg | $397.00 | |||
OSR1 CRISPR Activation Plasmid (h2) | sc-406034-ACT-2 | 20 µg | $397.00 |
Human OSR1 (odd-skipped related transcription factor 1) encodes a zinc finger DNA-binding protein that regulates cell fate decisions and patterning programs during embryonic development, with prominent roles in intermediate mesoderm specification and organogenesis. OSR1 functions as a transcriptional regulator that interfaces with morphogen-driven gene networks, including pathways influenced by WNT and BMP signaling, to control differentiation, migration, and tissue morphogenesis. Altered OSR1 expression has been associated with developmental abnormalities and dysregulated lineage programs in disease-relevant contexts, making it a useful node for studying transcriptional control of cell identity. In vitro, OSR1 is commonly investigated in models of epithelial differentiation and organoid development where transcription factor dosage can reshape regulatory circuits.
OSR1 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous OSR1 expression without altering the underlying DNA sequence.
OSR1 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the OSR1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the OSR1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous OSR1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native OSR1 locus and enabling the study of OSR1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of OSR1 pathway restoration in tumor cells with silenced or reduced OSR1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.