ORP150 CRISPR/Cas9 KO Plasmid (m): sc-419398
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ORP150 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ORP150 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ORP150 Antibody (A-3): sc-398224
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORP150 CRISPR/Cas9 KO Plasmid (m) | sc-419398 | 20 µg | $397.00 |
Overview
Hyou1 encodes ORP150 (also known as hypoxia upregulated protein 1), an endoplasmic reticulum–resident HSP70 family chaperone that supports protein folding and quality control during hypoxia, glucose deprivation, and other cellular stresses. ORP150 functions within ER proteostasis networks, coordinating with unfolded protein response pathways such as PERK–eIF2α, IRE1–XBP1, and ATF6 to limit accumulation of misfolded proteins and maintain secretory pathway integrity. In mouse models and cell systems, altered ORP150 activity has been linked to stress adaptation mechanisms affecting cell survival, inflammation, and metabolic homeostasis. Dysregulation of ER stress handling and proteostasis is relevant to studies of ischemic injury, neurodegeneration, and tumor microenvironment biology, where hypoxia and proteotoxic stress are prominent.
ORP150 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hyou1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hyou1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hyou1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ORP150 protein expression.
This CRISPR knockout system enables efficient generation of Hyou1-deficient cell models for investigation of ORP150 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hyou1 exon(s) critical for ORP150 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hyou1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ORP150 CRISPR/Cas9 KO Plasmid (m) and ORP150 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hyou1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ORP150 HDR Plasmid (m) and ORP150 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hyou1 homology arms to support homology-directed repair at defined Hyou1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.