OPN1SW Double Nickase Plasmid (h): sc-400755-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OPN1SW Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OPN1SW Double Nickase Plasmid (h) and OPN1SW Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OPN1SW. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OPN1SW Double Nickase Plasmid (h) | sc-400755-NIC | 20 µg | $410.00 | |||
OPN1SW Double Nickase Plasmid (h2) | sc-400755-NIC-2 | 20 µg | $410.00 |
OPN1SW encodes short-wavelength-sensitive opsin 1, a cone photopigment that binds 11-cis-retinal to form the blue-sensitive visual pigment in retinal cone outer segments. Upon photon absorption, OPN1SW activates the cone phototransduction cascade via transducin and phosphodiesterase signaling, lowering cGMP to modulate cyclic nucleotide–gated channels and shape membrane hyperpolarization. This pathway interfaces with outer segment trafficking, chromophore regeneration, and light-adaptation processes coordinated by retinal pigment epithelium support. Genetic variation or dysregulation affecting OPN1SW function has been linked to cone spectral sensitivity differences and can contribute to color vision phenotypes relevant to inherited retinal disorder research.
OPN1SW Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OPN1SW locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OPN1SW. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OPN1SW function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OPN1SW-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.