OKL38 CRISPR/Cas9 KO Plasmid (h): sc-405178
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OKL38 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OKL38 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OKL38 CRISPR/Cas9 KO Plasmid (h) | sc-405178 | 20 µg | $397.00 |
Overview
OSGIN1 encodes OKL38, an oxidative stress–induced protein implicated in growth inhibition and cellular stress adaptation. OKL38 is linked to redox homeostasis and p53-associated stress responses, with reported roles in regulating cell-cycle progression, apoptosis, and mitochondrial function under damaging conditions. Altered OSGIN1/OKL38 expression has been observed in contexts of epithelial transformation and tumor biology, where dysregulated stress signaling and mitochondrial pathways contribute to disease mechanisms. As a stress-responsive node, OSGIN1 provides a useful handle for dissecting how oxidative cues shape transcriptional programs and cell fate decisions.
OKL38 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OSGIN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OSGIN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OSGIN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OKL38 protein expression.
This CRISPR knockout system enables efficient generation of OSGIN1-deficient cell models for investigation of OKL38 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OSGIN1 exon(s) critical for OKL38 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OSGIN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OKL38 CRISPR/Cas9 KO Plasmid (h) and OKL38 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OSGIN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OKL38 HDR Plasmid (h) and OKL38 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OSGIN1 homology arms to support homology-directed repair at defined OSGIN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.