Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ODC CRISPR/Cas9 KO Plasmid (h): sc-401055
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ODC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ODC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ODC Antibody (E-6): sc-398116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ODC CRISPR/Cas9 KO Plasmid (h) | sc-401055 | 20 µg | $397.00 |
Overview
ODC1 encodes ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis that converts ornithine to putrescine, supporting production of spermidine and spermine required for DNA replication, chromatin organization, and translation. ODC activity is tightly controlled by antizyme-mediated degradation and integrates with amino acid metabolism, mTOR-linked growth signaling, and cell-cycle progression. Dysregulated polyamine metabolism and elevated ODC1 expression are frequently associated with proliferative phenotypes, metabolic adaptation, and oncogenic transcriptional programs, making ODC1 a useful node for studying growth control and stress responses. ODC1 perturbation also informs research on apoptosis, oxidative stress buffering, and immune or inflammatory signaling where polyamines modulate gene expression and cellular fitness.
ODC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ODC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ODC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ODC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ODC protein expression.
This CRISPR knockout system enables efficient generation of ODC1-deficient cell models for investigation of ODC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ODC1 exon(s) critical for ODC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ODC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ODC CRISPR/Cas9 KO Plasmid (h) and ODC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ODC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ODC HDR Plasmid (h) and ODC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ODC1 homology arms to support homology-directed repair at defined ODC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.