O-GlcNAc transferase Antibody (A-6): sc-74547
- O-GlcNAc transferase Antibody (A-6) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of O-GlcNAc transferase of human origin
- recommended for detection of O-GlcNAc transferase of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- See O-GlcNAc transferase (F-12): sc-74546 for O-GlcNAc transferase antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for O-GlcNAc transferase Antibody (A-6) for WB applications. This reagent is now offered in a bundle with O-GlcNAc transferase Antibody (A-6) (see ordering information below).
QUICK LINKS
SEE ALSO...
O-GlcNAc transferase Antibody (A-6) is a mouse monoclonal IgG2a antibody that detects O-GlcNAc transferase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). O-GlcNAc transferase plays a crucial role in cellular signaling by catalyzing the addition of a single N-acetylglucosamine moiety to serine or threonine residues, a process that can significantly influence protein function and interactions. This modification is particularly important as it competes with phosphorylation for the same residues, thereby modulating various signaling pathways and cellular responses. O-GlcNAc transferase is predominantly expressed in the pancreas, and O-GlcNAc transferase′s presence in both the nucleus and cytosol highlights involvement in diverse cellular processes. Notably, O-GlcNAc transferase exists as a heterotrimeric complex, which may affect enzymatic activity and interactions with other proteins. Anti-O-GlcNAc transferase antibody (A-6)′s ability to detect O-GlcNAc transferase across different species makes this product an invaluable tool for researchers studying the dynamics of glycosylation and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
O-GlcNAc transferase Antibody (A-6) References:
- O-GlcNAc in cancer biology. | Ma, Z. and Vosseller, K. 2013. Amino Acids. 45: 719-33. PMID: 23836420
- O-GlcNAc cycling and the regulation of nucleocytoplasmic dynamics. | Eustice, M., et al. 2017. Biochem Soc Trans. 45: 427-436. PMID: 28408483
- O-GlcNAc as an Integrator of Signaling Pathways. | Ong, Q., et al. 2018. Front Endocrinol (Lausanne). 9: 599. PMID: 30464755
- A nexus of lipid and O-Glcnac metabolism in physiology and disease. | Lockridge, A. and Hanover, JA. 2022. Front Endocrinol (Lausanne). 13: 943576. PMID: 36111295
- Integration of O-GlcNAc into Stress Response Pathways. | Fahie, KMM., et al. 2022. Cells. 11: PMID: 36359905
- Exploiting O-GlcNAc transferase promiscuity to dissect site-specific O-GlcNAcylation. | Mitchell, CW., et al. 2023. Glycobiology. 33: 1172-1181. PMID: 37856504
- Understanding and exploiting the roles of O-GlcNAc in neurodegenerative diseases. | Pratt, MR. and Vocadlo, DJ. 2023. J Biol Chem. 299: 105411. PMID: 37918804
- Cardioprotective O-GlcNAc signaling is elevated in murine female hearts via enhanced O-GlcNAc transferase activity. | Narayanan, B., et al. 2023. J Biol Chem. 299: 105447. PMID: 37949223
- O-GlcNAc regulates the mitochondrial integrated stress response by regulating ATF4. | Alghusen, IM., et al. 2023. Front Aging Neurosci. 15: 1326127. PMID: 38192280
- O-GlcNAc forces an α-synuclein amyloid strain with notably diminished seeding and pathology. | Balana, AT., et al. 2024. Nat Chem Biol. 20: 646-655. PMID: 38347213
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
O-GlcNAc transferase Antibody (A-6) | sc-74547 | 200 µg/ml | $322.00 | |||
O-GlcNAc transferase Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-539543 | 200 µg Ab; 10 µg BP | $361.00 |