Nup88 Double Nickase Plasmid (h): sc-404091-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nup88 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nup88 Double Nickase Plasmid (h) and Nup88 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NUP88. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nup88 Antibody (H-7): sc-365868
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup88 Double Nickase Plasmid (h) | sc-404091-NIC | 20 µg | $410.00 | |||
Nup88 Double Nickase Plasmid (h2) | sc-404091-NIC-2 | 20 µg | $410.00 |
NUP88 encodes Nup88, a core component of the cytoplasmic face of the nuclear pore complex that helps organize nucleocytoplasmic transport through interactions with nucleoporins such as NUP214 and transport receptors. By regulating mRNA and protein trafficking, Nup88 influences cell-cycle progression, stress responses, and signaling outputs that depend on timely nuclear import/export, including pathways linked to transcriptional control. Altered NUP88 expression or function has been associated with dysregulated nuclear transport and cellular homeostasis in cancer biology and developmental disease contexts. As a result, NUP88 is frequently studied in mechanisms connecting nuclear pore architecture to proteostasis and gene-expression programs.
Nup88 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NUP88 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NUP88. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NUP88 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NUP88-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.