Nrf1 Double Nickase Plasmid (h): sc-401053-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nrf1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nrf1 Double Nickase Plasmid (h) and Nrf1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFE2L1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nrf1 Antibody (G-5): sc-515360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nrf1 Double Nickase Plasmid (h) | sc-401053-NIC | 20 µg | $410.00 | |||
Nrf1 Double Nickase Plasmid (h2) | sc-401053-NIC-2 | 20 µg | $410.00 |
NFE2L1 encodes the transcription factor Nrf1, a membrane-tethered CNC-bZIP regulator that integrates proteostasis with cellular redox homeostasis. Upon proteasome stress, Nrf1 undergoes ER-associated processing and translocates to the nucleus to induce proteasome subunit genes and other detoxification programs via antioxidant response elements, coordinating ubiquitin–proteasome system capacity, lipid metabolism, and stress adaptation. Nrf1 signaling intersects with NRF2/KEAP1, unfolded protein response, and metabolic pathways that shape inflammatory and oxidative injury responses. Dysregulation of Nrf1-dependent transcription has been linked to altered proteasome function, metabolic imbalance, and context-dependent contributions to oncogenic and neurodegenerative phenotypes, making it relevant for mechanistic studies of protein quality control.
Nrf1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFE2L1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFE2L1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFE2L1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFE2L1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.