Nolz-1 CRISPR/Cas9 KO Plasmid (h): sc-405058
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nolz-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nolz-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nolz-1 CRISPR/Cas9 KO Plasmid (h) | sc-405058 | 20 µg | $397.00 |
Overview
ZNF503 (Nolz-1) encodes a zinc-finger transcription factor that regulates cell fate decisions by modulating lineage-specific gene expression programs during development and tissue patterning. Nolz-1 activity influences transcriptional networks linked to differentiation, proliferation control, and morphogen-driven processes, including retinoic acid–responsive gene regulation. Dysregulated expression or altered regulatory context of ZNF503 has been associated with changes in epithelial–mesenchymal traits and context-dependent tumor biology, making it relevant for studying transcriptional control of cellular identity. As a nuclear DNA-binding protein, Nolz-1 provides a tractable node for dissecting upstream signaling inputs and downstream gene regulatory circuits.
Nolz-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZNF503 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZNF503 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZNF503 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nolz-1 protein expression.
This CRISPR knockout system enables efficient generation of ZNF503-deficient cell models for investigation of Nolz-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZNF503 exon(s) critical for Nolz-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZNF503 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nolz-1 CRISPR/Cas9 KO Plasmid (h) and Nolz-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZNF503 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nolz-1 HDR Plasmid (h) and Nolz-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZNF503 homology arms to support homology-directed repair at defined ZNF503 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.