NOD1 CRISPR/Cas9 KO Plasmid (m): sc-430738
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NOD1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NOD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NOD1 Antibody (B-4): sc-398696
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOD1 CRISPR/Cas9 KO Plasmid (m) | sc-430738 | 20 µg | $397.00 |
Overview
Mouse Nod1 encodes NOD1, a cytosolic NOD-like receptor that detects specific diaminopimelic acid–containing peptidoglycan motifs from Gram-negative bacteria and initiates innate immune signaling. Upon ligand recognition, NOD1 recruits RIPK2 to activate NF-κB and MAPK pathways, promoting inflammatory gene expression, antimicrobial responses, and crosstalk with autophagy and epithelial barrier programs. NOD1 activity shapes host–microbiome interactions and influences cytokine production, making it relevant to experimental models of mucosal inflammation, infection biology, and immune-driven tissue injury. Dysregulated NOD1 signaling has been implicated in inflammatory pathophysiology and metabolic inflammation, supporting its use as a genetic node to interrogate innate immune circuitry.
NOD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nod1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nod1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nod1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NOD1 protein expression.
This CRISPR knockout system enables efficient generation of Nod1-deficient cell models for investigation of NOD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nod1 exon(s) critical for NOD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nod1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NOD1 CRISPR/Cas9 KO Plasmid (m) and NOD1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nod1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NOD1 HDR Plasmid (m) and NOD1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nod1 homology arms to support homology-directed repair at defined Nod1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.