NKD1 CRISPR/Cas9 KO Plasmid (h): sc-406642
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NKD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NKD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NKD1 CRISPR/Cas9 KO Plasmid (h) | sc-406642 | 20 µg | $397.00 |
Overview
NKD1 (naked cuticle homolog 1) encodes a cytoplasmic feedback regulator of Wnt/β-catenin signaling that binds Dishevelled proteins to attenuate pathway activation. By modulating β-catenin–dependent transcriptional programs, NKD1 influences cell fate decisions, epithelial differentiation, and tissue homeostasis. Altered NKD1 expression or function has been associated with dysregulated Wnt signaling in cancer-related contexts, including effects on proliferation, migration, and pathway-driven transcriptional signatures. As a Wnt pathway modulator, NKD1 is frequently studied in models of oncogenic signaling, developmental biology, and epithelial cell regulation.
NKD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NKD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NKD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NKD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NKD1 protein expression.
This CRISPR knockout system enables efficient generation of NKD1-deficient cell models for investigation of NKD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NKD1 exon(s) critical for NKD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NKD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NKD1 CRISPR/Cas9 KO Plasmid (h) and NKD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NKD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NKD1 HDR Plasmid (h) and NKD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NKD1 homology arms to support homology-directed repair at defined NKD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.