Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nix Double Nickase Plasmid (h): sc-401982-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nix Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nix Double Nickase Plasmid (h) and Nix Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BNIP3L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nix Antibody (H-8): sc-166332
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nix Double Nickase Plasmid (h) | sc-401982-NIC | 20 µg | $410.00 | |||
Nix Double Nickase Plasmid (h2) | sc-401982-NIC-2 | 20 µg | $410.00 |
BNIP3L encodes Nix, a mitochondrial outer membrane BH3-only protein that integrates hypoxia and cellular stress signals with programmed mitochondrial turnover. Nix promotes selective autophagy of mitochondria via LC3-interacting regions and cooperates with PINK1/Parkin-independent mitophagy programs, supporting mitochondrial quality control during differentiation and stress adaptation. Through regulation of mitochondrial membrane potential, ROS homeostasis, and BCL2 family interactions, Nix influences intrinsic apoptosis and metabolic remodeling. Dysregulated BNIP3L/Nix activity has been linked to altered mitophagy and cell fate decisions in contexts such as neurodegeneration, cardiometabolic stress, and cancer biology, making it a useful node for studying mitochondrial surveillance pathways.
Nix Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BNIP3L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BNIP3L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BNIP3L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BNIP3L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.