Na+ CP type Iβ CRISPR/Cas9 KO Plasmid (m): sc-422819
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Na+ CP type Iβ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Na+ CP type Iβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Na+ CP type Iβ CRISPR/Cas9 KO Plasmid (m) | sc-422819 | 20 µg | $397.00 |
Overview
Scn1b encodes the sodium channel β1 subunit (Na+ CP type Iβ), an auxiliary component that modulates voltage-gated sodium channel gating, cell-surface expression, and channel complex stability in excitable membranes. Beyond shaping action potential initiation and propagation, SCN1B participates in neuron–glia interactions and cell adhesion processes that influence network excitability and myelinated axon function. In mouse, Scn1b activity is tightly linked to pathways governing membrane excitability, synaptic transmission, and seizure susceptibility, and altered β1 function has been associated with epilepsy and broader neurodevelopmental phenotypes. These properties make Scn1b a useful target for dissecting mechanisms that connect ion channel regulation to circuit-level excitability and neurological disease models.
Na+ CP type Iβ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scn1b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scn1b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scn1b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Na+ CP type Iβ protein expression.
This CRISPR knockout system enables efficient generation of Scn1b-deficient cell models for investigation of Na+ CP type Iβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scn1b exon(s) critical for Na+ CP type Iβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scn1b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Na+ CP type Iβ CRISPR/Cas9 KO Plasmid (m) and Na+ CP type Iβ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scn1b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Na+ CP type Iβ HDR Plasmid (m) and Na+ CP type Iβ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scn1b homology arms to support homology-directed repair at defined Scn1b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.