Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MRG1 CRISPR/Cas9 KO Plasmid (m): sc-421714
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRG1 Antibody (JA22): sc-21795
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRG1 CRISPR/Cas9 KO Plasmid (m) | sc-421714 | 20 µg | $397.00 |
Overview
Cited2 encodes the transcriptional co-regulator MRG1, a nuclear protein that binds CBP/p300 and modulates gene expression programs controlling proliferation, differentiation, and cellular stress responses in mouse cells. By integrating signals from hypoxia-responsive and developmental transcription factors, MRG1 influences HIF-dependent transcriptional outputs and broader chromatin-mediated regulation of lineage specification. Cited2 activity is linked to embryogenesis and organogenesis, and altered regulation of this pathway has been associated with defects in cardiovascular and hematopoietic development. As a context-dependent regulator of transcriptional networks, Cited2 is frequently studied in models of inflammation, metabolic stress, and tumor biology where co-activator availability and hypoxic signaling shape cell fate decisions.
MRG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cited2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cited2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cited2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRG1 protein expression.
This CRISPR knockout system enables efficient generation of Cited2-deficient cell models for investigation of MRG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cited2 exon(s) critical for MRG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cited2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRG1 CRISPR/Cas9 KO Plasmid (m) and MRG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cited2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRG1 HDR Plasmid (m) and MRG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cited2 homology arms to support homology-directed repair at defined Cited2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.