MYL9/MYL12A/B Antibody (D-9): sc-48414
- MYL9/MYL12A/B Antibody (D-9) is a mouse monoclonal IgG1 κ, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-172 representing full length MRCL3 of human origin
- recommended for detection of the myosin regulatory light chains encoded by MYL9, MYL12A and MYL12B, and the LOC391722 protein of human origin; Mylc2b, Myl9 and Myl12a of mouse origin; and Mrlcb and Myl9 of rat origin by WB, IP, IF, IHC(P) and ELISA
- See MYL9/MYL12A/B (E-4): sc-28329 for MYL9/MYL12A/B antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MYL9/MYL12A/B Antibody (D-9) for WB and IHC(P) applications. These reagents are now offered in bundles with MYL9/MYL12A/B Antibody (D-9) (see ordering information below).
MYL9/MYL12A/B Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects MYL9 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MYL9, also known as LC20, MLC2, MRLC1, or MYRL2, plays a crucial role in muscle contraction regulation by interacting with myosin heavy chains and actin filaments. MYL9 is primarily located in the cytoplasm of smooth muscle and non-muscle cells, where MYL9 is essential for the contractile function. Phosphorylation of MYL9 by myosin light chain kinase (MLCK) in the presence of calcium and calmodulin enhances the actin-activated myosin ATPase activity, which is vital for muscle contraction and cellular motility. Additionally, MYL9 is found in striated skeletal muscle, where MYL9′s precise function is still being elucidated, highlighting MYL9′s importance in both smooth and striated muscle physiology. MYL9/MYL12A/B (D-9) antibody provides a reliable tool for researchers studying the dynamics of muscle contraction and the regulatory mechanisms involved in cellular movement.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MYL9/MYL12A/B Antibody (D-9) References:
- Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cells. | Aoki, T., et al. 2012. J Smooth Muscle Res. 48: 137-47. PMID: 23538510
- Crucial role for CD69 in allergic inflammatory responses: CD69-Myl9 system in the pathogenesis of airway inflammation. | Kimura, MY., et al. 2017. Immunol Rev. 278: 87-100. PMID: 28658550
- N-terminal acetylation and methylation differentially affect the function of MYL9. | Nevitt, C., et al. 2018. Biochem J. 475: 3201-3219. PMID: 30242065
- ITPRIP promotes glioma progression by linking MYL9 to DAPK1 inhibition. | Cao, C., et al. 2021. Cell Signal. 85: 110062. PMID: 34111521
- CD4+ T cells in inflammatory diseases: pathogenic T-helper cells and the CD69-Myl9 system. | Nakayama, T., et al. 2021. Int Immunol. 33: 699-704. PMID: 34427648
- Clinicopathological significance of MYL9 expression in pancreatic ductal adenocarcinoma. | Matsushita, K., et al. 2022. Cancer Rep (Hoboken). 5: e1582. PMID: 34821071
- Elevated Myl9 reflects the Myl9-containing microthrombi in SARS-CoV-2-induced lung exudative vasculitis and predicts COVID-19 severity. | Iwamura, C., et al. 2022. Proc Natl Acad Sci U S A. 119: e2203437119. PMID: 35895716
- LAMB3 Promotes Myofibrogenesis and Cytoskeletal Reorganization in Endometrial Stromal Cells via the RhoA/ROCK1/MYL9 Pathway. | Qin, X., et al. 2024. Cell Biochem Biophys. 82: 127-137. PMID: 37801199
- MYL9 promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis. | Wen, B., et al. 2023. J Int Med Res. 51: 3000605231208582. PMID: 37950670
- MYL9 binding with MYO19 suppresses epithelial-mesenchymal transition in non-small-cell lung cancer. | Sheng, M., et al. 2025. Physiol Genomics. 57: 1-7. PMID: 39437553
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYL9/MYL12A/B Antibody (D-9) | sc-48414 | 200 µg/ml | $322.00 | |||
MYL9/MYL12A/B Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-536835 | 200 µg Ab; 10 µg BP | $361.00 | |||
MYL9/MYL12A/B Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-534002 | 200 µg Ab; 40 µg BP | $361.00 | |||
MYL9/MYL12A/B Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-544849 | 200 µg Ab; 20 µg BP | $361.00 |