Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MPST CRISPR/Cas9 KO Plasmid (h): sc-401643
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MPST CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MPST genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MPST Antibody (H-11): sc-376168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MPST CRISPR/Cas9 KO Plasmid (h) | sc-401643 | 20 µg | $397.00 |
Overview
Human MPST (3-mercaptopyruvate sulfurtransferase) is a mitochondrial and cytosolic sulfurtransferase that catalyzes conversion of 3-mercaptopyruvate to pyruvate while generating reactive sulfur species and contributing to endogenous hydrogen sulfide (H2S) and persulfide metabolism. MPST functions in cellular redox homeostasis, sulfur amino acid utilization, and regulation of protein persulfidation, with downstream effects on mitochondrial bioenergetics and stress-response signaling. Altered MPST activity has been linked to oxidative stress sensitivity and metabolic dysregulation, and changes in MPST expression or sulfur signaling are studied in contexts such as neurobiology, cardiovascular signaling, and cancer cell adaptation. As part of the broader transsulfuration and reactive sulfur network, MPST provides a mechanistic entry point to dissect thiol-dependent signaling and mitochondrial function.
MPST CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MPST gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MPST together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MPST open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MPST protein expression.
This CRISPR knockout system enables efficient generation of MPST-deficient cell models for investigation of MPST signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MPST exon(s) critical for MPST function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MPST genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MPST CRISPR/Cas9 KO Plasmid (h) and MPST CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MPST locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MPST HDR Plasmid (h) and MPST HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MPST homology arms to support homology-directed repair at defined MPST target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.