MMP-2 Anticuerpo (2C1) recomendado para detectarMMP-2 de mouse, rat y human origen, mediante WB, IP y IF

MMP-2 Anticuerpo (2C1): sc-13594

MMP-2 Anticuerpo (2C1) clasificado 4.3 de 5 por 41.
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5
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Ficha técnica

    • Anti-MMP-2 Anticuerpo 2C1 es un monoclonal de ratón IgG1 (kappa light chain) MMP-2 Anticuerpo proporcionado como 200 µg/ml
    • raised against the activated form of MMP-2 of human origin
    • MMP-2 Anticuerpo (2C1) es recomendado para detectar MMP-2 de mouse, rat y human origen, mediante WB, IP y IF
    • MMP-2 Anticuerpo (2C1) es disponible conjugado a agarosa para IP; y tanto a ficoeritina como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de MMP-2 (2C1): sc-13594.

Does it work for bovine tissue?

Preguntado por: Anónimo
Thank you for your question. This antibody has not yet been tested in bovine samples and so that is not a recommended species at this time. We are still gathering data to show on our website. We do have a previous citation in bovine samples which may be helpful for you: PMID: 12844104.
Respondida por: Tech Service
Fecha de publicación: 2019-11-20

We are looking for a MMP-2 antibody that works well in Western blotting . For this we would like to use a 10ug free sample of MMP2 antibody (2C1): sc-13594. I was wondering if we could get some.

Preguntado por: Shiv
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Respondida por: Technical Support
Fecha de publicación: 2019-02-26

Hello.. is there a recommended buffer for use of this product with tissue lysates and immunoblotting? We usually use RIPA buffer for protein expression, but was concerned about chelators.

Preguntado por: Nabilb
Thank you for your question. We do not have any specific buffer recommendation for use with this MMP-2 antibody and have prepared our lysates for testing using our standard RIPA buffer. You may want to refer to some of the numerous product citations using this antibody for Western blotting to see if they have any optimized recommendations.
Respondida por: Technical Support
Fecha de publicación: 2019-01-26

What is the epitope sequence (binding site) of this antibody? Could you tell me a.a. number?

Preguntado por: neoSong
Thank you for your question. This mouse monoclonal antibody, MMP-2 (2C1): sc-13594, is raised against the activated form of MMP-2 of human origin. The exact epitope for this monoclonal antibody has not yet been determined.
Respondida por: Technical Support
Fecha de publicación: 2017-09-14

Can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Preguntado por: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This monoclonal for MMP-2 is available conjugated to agarose (sc-13594 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-13594 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-13594 PE), fluorescein (sc-13594 FITC), Alexa Fluor® 488 (sc-13594 AF488) or Alexa Fluor® 647 (sc-13594 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Respondida por: Technical Support 13
Fecha de publicación: 2017-01-04
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Rated 5 de 5 por de UPENN Gα 12 Antibody is very good. The band is very stronger.
Fecha de publicación: 2018-04-19
Rated 3 de 5 por de 抗体特异性还可以 用着效果还行。带的大小有些不确定,最上一条带在70kd以下,认为是剪切的mmp2,所以下面的条带不确定是什么。
Fecha de publicación: 2018-03-20
Rated 5 de 5 por de Great product MMP-2 antibody works well at the concentration of 1:500 with satisfied bands
Fecha de publicación: 2018-02-08
Rated 5 de 5 por de western blot This antibody generates a clearsignal with the HepG2 cell lysis (1:200).
Fecha de publicación: 2018-01-25
Rated 1 de 5 por de Unsucessful western blot with glioblastoma cells I have used this antibody 1:100-1:200 for western blotting and failled to obtain any band with the expected molecular weight using lysates from human glioblastoma cell lines
Fecha de publicación: 2017-11-28
Rated 2 de 5 por de May not be suitable for IF Sample:muscle cryosection Applications: IF 1:100, 1:200, 1:400 Results: Very weak staining. A little bit staining in nuclear. No difference with negetive control.
Fecha de publicación: 2017-11-22
Rated 1 de 5 por de Didnot work for WB did not work for western - samples were human cells in culture
Fecha de publicación: 2017-11-21
Rated 5 de 5 por de work well 一月前我向贵公司申请了该抗体的试用装,结果非常好,条带清楚明显,位置正确,抗体很不错,可靠有用,值得再次购买
Fecha de publicación: 2017-10-19
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