Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MKP-3 CRISPR/Cas9 KO Plasmid (h): sc-400850
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MKP-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MKP-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MKP-3 Antibody (F-12): sc-377070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-3 CRISPR/Cas9 KO Plasmid (h) | sc-400850 | 20 µg | $397.00 |
Overview
DUSP6 encodes the dual-specificity phosphatase MKP-3, a cytosolic MAPK phosphatase that preferentially dephosphorylates and inactivates ERK1/2 (MAPK3/MAPK1) to shape the magnitude and duration of RTK–RAS–RAF–MEK–ERK signaling. By providing negative feedback on ERK activity, MKP-3 influences cell proliferation, differentiation, and stress-response programs, and helps maintain signaling homeostasis in contexts driven by growth factor cues such as FGFR and EGFR pathways. Altered DUSP6 expression or activity has been linked to dysregulated MAPK signaling observed across multiple disease-relevant settings, including oncogenic pathway activation, inflammatory responses, and developmental phenotypes. As a pathway gatekeeper, MKP-3 is commonly studied for its role in feedback control, signaling adaptation, and ERK-dependent transcriptional outputs.
MKP-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DUSP6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DUSP6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DUSP6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MKP-3 protein expression.
This CRISPR knockout system enables efficient generation of DUSP6-deficient cell models for investigation of MKP-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DUSP6 exon(s) critical for MKP-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DUSP6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MKP-3 CRISPR/Cas9 KO Plasmid (h) and MKP-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DUSP6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MKP-3 HDR Plasmid (h) and MKP-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DUSP6 homology arms to support homology-directed repair at defined DUSP6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.