melanotransferrin Antibody (H-11): sc-271919

Melanotransferrin Antibody (H-11) is a mouse monoclonal IgG3 antibody that detects melanotransferrin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Melanotransferrin, also known as p97, is a member of the transferrin family of iron-binding proteins, which includes serum transferrin, lactoferrin, and ovotransferrin, and is notably expressed on melanoma cells. Anti-melanotransferrin antibody (H-11) recognizes a unique structure that allows binding to iron, albeit not as efficiently as transferrin. This structural characteristic suggests melanotransferrin may have specialized roles beyond iron transport, such as involvement in cellular processes like membrane integrity and protection against oxidative stress. The gene encoding human melanotransferrin is located on chromosome 3q28-29 and is predominantly expressed in either a membrane-bound form or as a secreted protein. Melanotransferrin is found in the brain, where melanotransferrin may play a role in neurodegenerative conditions such as Alzheimer′s disease. Melanotransferrin′s potential functions, including protection against membrane-lipid peroxidation and participation in intracellular adhesion, highlight the need for further research to fully understand the biological significance of this intriguing protein.

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melanotransferrin Antibody (H-11) References:

1. Melanotransferrin is produced by senile plaque-associated reactive microglia in Alzheimer's disease.  |  Yamada, T., et al. 1999. Brain Res. 845: 1-5. PMID: 10529437
2. Human melanotransferrin (p97) has only one functional iron-binding site.  |  Baker, EN., et al. 1992. FEBS Lett. 298: 215-8. PMID: 1544447
3. The function of melanotransferrin: a role in melanoma cell proliferation and tumorigenesis.  |  Dunn, LL., et al. 2006. Carcinogenesis. 27: 2157-69. PMID: 16704991
4. Melanotransferrin stimulates t-PA-dependent activation of plasminogen in endothelial cells leading to cell detachment.  |  Rolland, Y., et al. 2006. Biochim Biophys Acta. 1763: 393-401. PMID: 16713448
5. Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.  |  Shin, J., et al. 2014. J Proteome Res. 13: 4919-31. PMID: 25216327
6. Conjugation of melanotransferrin antibody on solid lipid nanoparticles for mediating brain cancer malignancy.  |  Kuo, YC. and Chao, IW. 2016. Biotechnol Prog. 32: 480-90. PMID: 26701338
7. Modulation of Melanotransferrin and Transferrin Receptor 1 (TFRC)- and CD44-based Signaling for TFRC Up-regulation in Human Melanoma Cells.  |  Laube, F. and Glanz, D. 2017. Anticancer Res. 37: 3001-3007. PMID: 28551638
8. The membrane-bound and soluble form of melanotransferrin function independently in the diagnosis and targeted therapy of lung cancer.  |  Lei, Y., et al. 2020. Cell Death Dis. 11: 933. PMID: 33127882
9. The Role of Melanotransferrin (CD228) in the regulation of the differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells (hBM-MSC).  |  Dubon, M., et al. 2021. Int J Med Sci. 18: 1580-1591. PMID: 33746574
10. Reactive microglia specifically associated with amyloid plaques in Alzheimer's disease brain tissue express melanotransferrin.  |  Jefferies, WA., et al. 1996. Brain Res. 712: 122-6. PMID: 8705294

The preferred melanotransferrin antibody is melanotransferrin Antibody (E-4): sc-271633.