Mel-CAM Antibody (P1H12): sc-18837

Mel-CAM Antibody (P1H12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mel-CAM protein of mouse, rat, human, canine, and rabbit origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-Mel-CAM antibody (P1H12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mel-CAM protein, also known as MCAM, MUC18, or CD146, is a transmembrane glycoprotein that plays a critical role in cell adhesion, particularly in melanoma. Mel-CAM′s structure includes a signal peptide, five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail, which are essential for function as a calcium-independent adhesion molecule. Mel-CAM is predominantly expressed in advanced primary and metastatic melanomas, with expression levels correlating strongly with metastatic disease progression. Expression of Mel-CAM occurs in 80% of advanced primary human melanomas, making Mel-CAM a significant marker for tumorigenic activity. Mel-CAM′s ability to initiate outside-in signaling pathways involving protein tyrosine kinases such as Fyn, FAK, and paxillin is crucial for influencing actin cytoskeleton dynamics, which affects cellular morphology and migration. Mel-CAM is essential for maintaining thymic architecture and function, highlighting Mel-CAM′s importance not only in cancer biology but also in immune system development.

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Mel-CAM Antibody (P1H12) References:

1. The role of CD146 (Mel-CAM) in biology and pathology.  |  Shih, IM. 1999. J Pathol. 189: 4-11. PMID: 10451481
2. Expression of CD146 adhesion molecules (MUC18 or MCAM) in the thymic microenvironment.  |  Seftalioğlu, A. and Karakoç, L. 2000. Acta Histochem. 102: 69-83. PMID: 10726166
3. Outside-in signaling pathway linked to CD146 engagement in human endothelial cells.  |  Anfosso, F., et al. 2001. J Biol Chem. 276: 1564-9. PMID: 11036077
4. Mel-CAM-specific genetic suppressor elements inhibit melanoma growth and invasion through loss of gap junctional communication.  |  Satyamoorthy, K., et al. 2001. Oncogene. 20: 4676-84. PMID: 11498790
5. Bioimmunotherapy for melanoma using fully human antibodies targeting MCAM/MUC18 and IL-8.  |  Melnikova, VO. and Bar-Eli, M. 2006. Pigment Cell Res. 19: 395-405. PMID: 16965268
6. MUC18, a marker of tumor progression in human melanoma, shows sequence similarity to the neural cell adhesion molecules of the immunoglobulin superfamily.  |  Lehmann, JM., et al. 1989. Proc Natl Acad Sci U S A. 86: 9891-5. PMID: 2602381
7. Mel-CAM Expression in Common Oral Carcinomas.  |  Shamloo, N., et al. 2020. J Dent (Shiraz). 21: 184-189. PMID: 33062811
8. Expression of MEL-CAM and HSD3B1 in cervical carcinoma.  |  Olusoji, MJ., et al. 2021. Pathol Int. 71: 561-563. PMID: 34157783
9. MCAM+ brain endothelial cells contribute to neuroinflammation by recruiting pathogenic CD4+ T lymphocytes.  |  Charabati, M., et al. 2023. Brain. 146: 1483-1495. PMID: 36319587
10. Mcam inhibits macrophage-mediated development of mammary gland through non-canonical Wnt signaling.  |  Yang, X., et al. 2024. Nat Commun. 15: 36. PMID: 38167296
11. Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.  |  Kuzu, I., et al. 1993. Lab Invest. 69: 322-8. PMID: 7690867
12. Genomic organization of the melanoma-associated glycoprotein MUC18: implications for the evolution of the immunoglobulin domains.  |  Sers, C., et al. 1993. Proc Natl Acad Sci U S A. 90: 8514-8. PMID: 8378324

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mel-CAM Antibody (P1H12) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-Mel-CAM Antibody (P1H12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mel-CAM Antibody (P1H12) Specifications

Alternate Product Name: CD146

Product Clone Name: P1H12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Mel-CAM

Epitope Species: human

Product Reactivity/Detection: Mel-CAM

Species Reactivity/Detection: mouse, rat, human, canine, rabbit

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for Mel-CAM Antibody (P1H12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin