Max Double Nickase Plasmid (h): sc-400596-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Max Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Max Double Nickase Plasmid (h) and Max Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Max Antibody (H-2): sc-8011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Max Double Nickase Plasmid (h) | sc-400596-NIC | 20 µg | $410.00 | |||
Max Double Nickase Plasmid (h2) | sc-400596-NIC-2 | 20 µg | $410.00 |
Human MAX encodes Max, a basic helix–loop–helix leucine zipper transcription factor that dimerizes with MYC family proteins to regulate E-box–dependent gene expression programs controlling cell growth, metabolism, ribosome biogenesis, and cell-cycle progression. Max also forms repressive complexes with MXD/MNT partners, providing a counterbalance to MYC-driven transcriptional activation and helping maintain transcriptional homeostasis. Through these context-dependent activating and repressive dimers, MAX functions within MYC/MAX/MAD network signaling and interfaces with chromatin regulation to shape lineage- and stimulus-specific transcriptional outputs. Altered MAX activity or disruption of MYC–MAX axis has been linked to dysregulated proliferation and oncogenic transcriptional states, supporting its study in tumor biology, metabolic adaptation, and transcriptional control.
Max Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.