Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LRP5 Double Nickase Plasmid (h): sc-401331-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRP5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LRP5 Double Nickase Plasmid (h) and LRP5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LRP5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRP5 Antibody (B-9): sc-390267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRP5 Double Nickase Plasmid (h) | sc-401331-NIC | 20 µg | $410.00 | |||
LRP5 Double Nickase Plasmid (h2) | sc-401331-NIC-2 | 20 µg | $410.00 |
LRP5 (low-density lipoprotein receptor-related protein 5) is a single-pass transmembrane co-receptor that partners with Frizzled receptors to transduce canonical Wnt/β-catenin signaling. By modulating β-catenin stabilization and TCF/LEF-dependent transcription, LRP5 regulates osteoblast activity, bone mass accrual, retinal vascular development, and broader programs of cell fate specification. LRP5 function intersects with extracellular Wnt modulators and antagonists, including DKK and sclerostin, linking receptor availability at the plasma membrane to downstream transcriptional outputs. Genetic variation or dysregulation of LRP5 is associated with altered bone density phenotypes and ocular vascular disorders, making it a widely used target for mechanistic studies of Wnt pathway control.
LRP5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LRP5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LRP5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LRP5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LRP5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.