LOXL2 Double Nickase Plasmid (m): sc-430162-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOXL2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LOXL2 Double Nickase Plasmid (m) and LOXL2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Loxl2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LOXL2 Antibody (3C5): sc-293427
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOXL2 Double Nickase Plasmid (m) | sc-430162-NIC | 20 µg | $410.00 |
Mouse Loxl2 encodes lysyl oxidase-like 2 (LOXL2), a copper-dependent amine oxidase that catalyzes oxidative deamination of lysine residues in collagen and elastin, promoting covalent crosslinking and extracellular matrix maturation. Through regulation of matrix stiffness and collagen fiber organization, LOXL2 influences cell adhesion, migration, and mechanotransduction pathways, including integrin/FAK signaling and EMT-associated transcriptional programs. Altered LOXL2 activity has been linked to fibrotic remodeling and tumor microenvironment dynamics, making it relevant for studies of tissue architecture, invasion biology, and stromal–epithelial interactions. In mouse models, Loxl2 perturbation is commonly used to dissect how extracellular matrix remodeling shapes inflammation, wound repair, and metastatic niche formation.
LOXL2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Loxl2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Loxl2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Loxl2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Loxl2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.