Anti-LIP5 Anticuerpo D-6 es un monoclonal de ratón IgG1 (kappa light chain) LIP5 Anticuerpo proporcionado como 200 µg/ml
producido contra los amino ácidos 1-300 localizados en el N-terminus de origen LIP5 of human
LIP5 Anticuerpo (D-6) es recomendado para detectar LIP5 of human origin and Vta1 of mouse and rat origin de mouse, rat y human origen, mediante WB, IP, IF y ELISA
LIP5 Anticuerpo (D-6) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de LIP5 (D-6): sc-374012.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for LIP5 Antibody (D-6) for WB applications. This reagent is now offered in a bundle with LIP5 Antibody (D-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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LIP5 Antibody (D-6) is a high quality monoclonal LIP5 antibody (also designated LIP5 antibody) suitable for the detection of the LIP5 protein of mouse, rat and human origin. LIP5 Antibody (D-6) is available as both the non-conjugated anti-LIP5 antibody form, as well as multiple conjugated forms of anti-LIP5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. LIP5 (LYST-interacting protein 5), also known as VTA1 (Vps20-associated 1 homolog), DRG1 (dopamine-responsive protein DRG-1), SBP1 (SKD1 binding protein 1) or My012, is a 307 amino acid protein belonging to the VTA1 family. LIP5 plays a role in sorting membrane proteins, including lysosomal enzymes, lipids and stimulated growth factor receptors, for lysosomal degradation in a pathway known as the endosomal multivesicular bodies (MVB) pathway. Found in brain, liver, heart and kidney, LIP5 localizes to cytoplasm and both endosomal and peripheral membranes. RNAi studies demonstrate that LIP5 depletion decreases human immunodeficiency virus type 1 (HIV-1) budding, and LIP5 is known to interact with CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1) and possibly CHMP3.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with LIP5 (D-6): sc-374012 monoclonal antibody?
Preguntado por: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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