LIME Antibody (H-8): sc-376536

LIME Antibody (H-8) is a mouse monoclonal IgG1 antibody that detects LIME in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-LIME antibody (H-8) recognizes LIME, a 295 amino acid transmembrane adaptor protein predominantly expressed in hematopoietic and lung cells, which plays a crucial role in T cell activation and signaling. LIME features a short extracellular domain and a cytoplasmic tail rich in five tyrosine-based motifs, which are essential for its function. Upon cross-linking of the CD4 or CD8 co-receptors, LIME undergoes tyrosine phosphorylation, enabling interaction with Lck, a member of the Src family kinases, and Csk, its negative regulator. This interaction is vital for the regulation of T cell activation, as LIME is involved in the activation of key signaling pathways, including the ERK and JNK pathways, which are critical for T cell responses. Additionally, LIME may also play a role in B cell receptor-mediated B cell activation, highlighting its importance in the immune response. LIME (H-8) antibody is an invaluable tool for researchers studying T cell dynamics and signaling pathways in human immunology.

The preferred LIME antibody is LIME Antibody (A-7): sc-365195.