Laminin alpha-4 Anticuerpo (6C3) recomendado para detectar Laminin α-4 de human origen, mediante WB, IP y ELISA

Laminin α-4 Anticuerpo (6C3): sc-130541

Laminin α-4 Anticuerpo (6C3) clasificado 3.0 de 5 por 4.
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Ficha técnica

    • Anti-Laminin alpha-4 Anticuerpo 6C3 es un monoclonal de ratón IgG1 (kappa light chain) Laminin α-4 Anticuerpo proporcionado como 100 µg/ml
    • raised against purified Laminin α4β1γ1 isolated from platelets of human origin
    • recomendado para detectar Laminin α-4 de human origen, mediante WB, IP y ELISA
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Laminin α-4 (6C3): sc-130541.
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.

For Western Blot, is it recommended to use denatured or non-denatured conditions with Laminin α-4 (6C3): sc-130541 antibody?

Preguntado por: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Respondida por: Technical Support
Fecha de publicación: 2017-03-27
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Rated 1 de 5 por de Did not work for western blot Used a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. No band observed.
Fecha de publicación: 2017-11-30
Rated 4 de 5 por de Staining of human RCC paraffin embedded tissue The antibody works well on human paraffin sections of renal cell carcinoma. Matching normal kidney tissue of the same RCC patient shows some Lama4 staining. Protocol After deparaffinization with xylene and ethanol block endogenous peroxidase with 0.3% hydrogen peroxidase/PBS 15 min at RT. Antigen retrieval with 10mM citrate buffer pH 6.0, block 5% BSA/PBS 1h at RT, anti-lama4 Ab dilution 1:30 in 0.5% BSA/PBS O.N. at 4C, 2nd Ab goat anti-mouse biotin (Dako, ref E0433) diution 1:500 in 0.5% BSA/PBS 45 min at RT. Strep-HRP (Dako, ref P0397) 1:200 in 0.5% BSA/PBS 30 min at RT. Develop with DAB, counterstain with Mayer's hematoxylin.
Fecha de publicación: 2017-11-23
Rated 5 de 5 por de Good results in IF of human kidney sections Staining with the antibody (1:100) resulted in good staining of murine kidney kryosections (PFA-fixed)
Fecha de publicación: 2017-11-17
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