Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KLK7 CRISPR/Cas9 KO Plasmid (h): sc-403539
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KLK7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KLK7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KLK7 Antibody (H-5): sc-514447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLK7 CRISPR/Cas9 KO Plasmid (h) | sc-403539 | 20 µg | $397.00 |
Overview
KLK7 encodes kallikrein-related peptidase 7, a secreted serine protease that contributes to epidermal homeostasis by processing components of the stratum corneum and modulating desquamation. KLK7 participates in extracellular proteolytic cascades and can influence barrier function, cell–cell adhesion, and inflammatory signaling through regulated cleavage of protein substrates in the skin microenvironment. Dysregulated KLK7 expression or activity has been associated with cutaneous inflammation and barrier defects, and is frequently investigated in epithelial malignancy contexts where pericellular proteolysis can affect invasion-related phenotypes. As a result, KLK7 is commonly studied in pathways linking protease activity to differentiation, cytokine networks, and extracellular matrix remodeling.
KLK7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KLK7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KLK7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KLK7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KLK7 protein expression.
This CRISPR knockout system enables efficient generation of KLK7-deficient cell models for investigation of KLK7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KLK7 exon(s) critical for KLK7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KLK7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KLK7 CRISPR/Cas9 KO Plasmid (h) and KLK7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KLK7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KLK7 HDR Plasmid (h) and KLK7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KLK7 homology arms to support homology-directed repair at defined KLK7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.