Keratin 24 CRISPR/Cas9 KO Plasmid (h): sc-414948
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Keratin 24 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Keratin 24 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Keratin 24 CRISPR/Cas9 KO Plasmid (h) | sc-414948 | 20 µg | $397.00 |
Overview
KRT24 encodes keratin 24, a type I intermediate filament protein that contributes to the cytoskeletal network in epithelial cells and supports tissue-specific structural integrity and mechanical resilience. As part of keratin filament assembly and remodeling, KRT24 integrates with broader cytoskeleton- and adhesion-linked processes that influence cell shape, polarity, and barrier function. Altered keratin expression patterns are commonly associated with epithelial stress responses and changes in differentiation state, making KRT24 a useful marker and mechanistic entry point for studying epithelial remodeling in disease-relevant contexts. Dysregulated intermediate filament organization can affect signaling cross-talk and cellular homeostasis, providing rationale to interrogate KRT24 in models of epithelial dysfunction.
Keratin 24 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KRT24 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KRT24 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KRT24 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Keratin 24 protein expression.
This CRISPR knockout system enables efficient generation of KRT24-deficient cell models for investigation of Keratin 24 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KRT24 exon(s) critical for Keratin 24 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KRT24 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Keratin 24 CRISPR/Cas9 KO Plasmid (h) and Keratin 24 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KRT24 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Keratin 24 HDR Plasmid (h) and Keratin 24 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KRT24 homology arms to support homology-directed repair at defined KRT24 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.