KDEL receptor 2 CRISPR/Cas9 KO Plasmid (m): sc-426263
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KDEL receptor 2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KDEL receptor 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KDEL receptor 2 CRISPR/Cas9 KO Plasmid (m) | sc-426263 | 20 µg | $397.00 |
Overview
Kdelr2 encodes KDEL receptor 2, a Golgi-resident retrieval receptor that recognizes C-terminal KDEL-like motifs on soluble ER luminal proteins and mediates their retrograde transport from the Golgi to the endoplasmic reticulum via COPI-coated vesicles. By maintaining ER proteostasis and proper compartmentalization of chaperones and folding enzymes, KDEL receptor 2 supports secretory pathway fidelity and limits stress signaling linked to ER quality control. This trafficking axis intersects with unfolded protein response regulation, glycoprotein maturation, and Golgi-to-ER recycling dynamics that influence cellular adaptation to proteotoxic stress. Dysregulation of ER–Golgi transport and proteostasis has been associated with neurodegeneration, metabolic dysfunction, and cancer cell secretory remodeling, making Kdelr2 a useful node for mechanistic studies.
KDEL receptor 2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Kdelr2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Kdelr2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Kdelr2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KDEL receptor 2 protein expression.
This CRISPR knockout system enables efficient generation of Kdelr2-deficient cell models for investigation of KDEL receptor 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Kdelr2 exon(s) critical for KDEL receptor 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Kdelr2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KDEL receptor 2 CRISPR/Cas9 KO Plasmid (m) and KDEL receptor 2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Kdelr2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KDEL receptor 2 HDR Plasmid (m) and KDEL receptor 2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Kdelr2 homology arms to support homology-directed repair at defined Kdelr2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.