Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin α2 Double Nickase Plasmid (h): sc-401484-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin α2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- karyopherin α2 Double Nickase Plasmid (h) and karyopherin α2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KPNA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin α2 Antibody (B-9): sc-55538
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin α2 Double Nickase Plasmid (h) | sc-401484-NIC | 20 µg | $410.00 | |||
karyopherin α2 Double Nickase Plasmid (h2) | sc-401484-NIC-2 | 20 µg | $410.00 |
KPNA2 encodes karyopherin alpha 2, an importin-α adaptor that recognizes classical nuclear localization signals and cooperates with importin-β to mediate Ran GTPase–regulated nucleocytoplasmic transport through the nuclear pore complex. By controlling nuclear entry of transcription factors, DNA repair proteins, and cell-cycle regulators, KPNA2 influences mitotic progression, genome stability, and stress-response signaling. Dysregulated KPNA2 expression has been linked to altered nuclear transport programs and proliferative phenotypes in multiple cancer contexts, making it a useful node for studying transport-dependent regulation of oncogenic signaling and chromatin-associated processes. KPNA2 function is also relevant to investigations of nuclear import dynamics, proteostasis, and context-dependent transcriptional control.
karyopherin α2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KPNA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KPNA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KPNA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KPNA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.