Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JMJD2B Double Nickase Plasmid (h): sc-405385-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD2B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JMJD2B Double Nickase Plasmid (h) and JMJD2B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KDM4B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD2B Antibody (F-12): sc-374241
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD2B Double Nickase Plasmid (h) | sc-405385-NIC | 20 µg | $410.00 | |||
JMJD2B Double Nickase Plasmid (h2) | sc-405385-NIC-2 | 20 µg | $410.00 |
Human KDM4B (JMJD2B) encodes a Jumonji C (JmjC) domain–containing histone lysine demethylase that primarily removes repressive H3K9me3/me2 marks, reshaping chromatin accessibility and transcriptional programs. Through its coupling to epigenetic control of cell-cycle progression, DNA damage responses, and lineage-associated gene expression, JMJD2B influences pathways that coordinate proliferation and differentiation. Dysregulated KDM4B activity and expression have been linked to aberrant transcriptional states observed across multiple disease-relevant contexts, including oncogenic signaling and altered hormone- or hypoxia-responsive networks. As a chromatin regulator, JMJD2B is frequently studied to define how histone methylation dynamics affect gene regulation and genome stability.
JMJD2B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KDM4B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KDM4B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KDM4B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KDM4B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.