Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IQGAP1 Double Nickase Plasmid (m): sc-424375-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IQGAP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IQGAP1 Double Nickase Plasmid (m) and IQGAP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Iqgap1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IQGAP1 Antibody (C-9): sc-376021
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IQGAP1 Double Nickase Plasmid (m) | sc-424375-NIC | 20 µg | $410.00 | |||
IQGAP1 Double Nickase Plasmid (m2) | sc-424375-NIC-2 | 20 µg | $410.00 |
Mouse Iqgap1 encodes IQGAP1, a multidomain scaffold protein that coordinates actin cytoskeleton remodeling with membrane trafficking and signal transduction. IQGAP1 binds small GTPases such as CDC42 and RAC1 and interfaces with β-catenin, calmodulin, and MAPK components to regulate cell polarity, adhesion, migration, and cytokinesis. Through these interactions it helps integrate pathways controlling junctional stability and dynamic cytoskeletal rearrangements, processes frequently studied in tissue morphogenesis and immune cell motility. Dysregulated IQGAP1-associated signaling and altered cell–cell adhesion programs are commonly investigated in models of inflammation, fibrosis, and cancer-related phenotypes, supporting its relevance in disease-mechanism research.
IQGAP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Iqgap1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Iqgap1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Iqgap1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Iqgap1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.