Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h): sc-404333-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h) and Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGAE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin αE/ITGAE/CD103 Antibody (Ber-ACT8): sc-19981
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h) | sc-404333-NIC | 20 µg | $410.00 | |||
Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h2) | sc-404333-NIC-2 | 20 µg | $410.00 |
ITGAE encodes integrin αE (CD103), which heterodimerizes with integrin β7 to form the αEβ7 adhesion receptor that binds E-cadherin and supports retention of lymphocytes within epithelial tissues. This receptor participates in integrin outside-in signaling that coordinates cytoskeletal remodeling, cell migration, and immune synapse dynamics, linking to pathways involving focal adhesion kinases and downstream MAPK and PI3K signaling. CD103 is a hallmark of tissue-resident memory T cells and subsets of dendritic cells, shaping mucosal immune surveillance and epithelial barrier interactions. Altered ITGAE expression and CD103-positive immune infiltrates are widely studied in chronic inflammatory settings and in tumor immune microenvironments, where they inform mechanisms of immune cell localization and function.
Integrin αE/ITGAE/CD103 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGAE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGAE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGAE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGAE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.