Integrin αE/ITGAE/CD103 CRISPR/Cas9 KO Plasmid (h): sc-404333

Overview

ITGAE encodes integrin αE (CD103), which heterodimerizes with integrin β7 to form αEβ7, an adhesion receptor that binds E-cadherin and supports retention of lymphocytes within epithelial tissues. This integrin contributes to immune cell trafficking, tissue residency, and immunological synapse organization, integrating extracellular matrix and cell–cell adhesion cues with cytoskeletal remodeling and outside-in signaling pathways that influence activation state. CD103 is widely used as a marker of tissue-resident memory T cells and subsets of dendritic cells, and its function intersects with pathways controlling epithelial barrier immunity and mucosal surveillance. Dysregulated αEβ7-mediated interactions have been associated with inflammatory and autoimmune processes in barrier tissues and with tumor immune microenvironment composition, making ITGAE a relevant target for mechanistic studies of immune infiltration and epithelial–immune crosstalk.

Integrin αE/ITGAE/CD103 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ITGAE gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ITGAE together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ITGAE open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin αE/ITGAE/CD103 protein expression.

This CRISPR knockout system enables efficient generation of ITGAE-deficient cell models for investigation of Integrin αE/ITGAE/CD103 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ITGAE exon(s) critical for Integrin αE/ITGAE/CD103 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ITGAE genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Integrin αE/ITGAE/CD103 CRISPR/Cas9 KO Plasmid (h) and Integrin αE/ITGAE/CD103 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ITGAE locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Integrin αE/ITGAE/CD103 HDR Plasmid (h) and Integrin αE/ITGAE/CD103 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ITGAE homology arms to support homology-directed repair at defined ITGAE target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.