Integrin β5/ITGB5 CRISPR/Cas9 KO Plasmid (m): sc-421177

Overview

Itgb5 encodes integrin β5, a transmembrane adhesion receptor that primarily pairs with integrin αV to form αVβ5, enabling cell attachment to extracellular matrix ligands such as vitronectin. Through focal adhesion assembly and bidirectional signaling, αVβ5 modulates cytoskeletal remodeling and activates pathways including FAK/Src, PI3K–AKT, and MAPK/ERK that coordinate migration, survival, and proliferation. ITGB5 also contributes to endocytic and mechanotransduction processes that influence tissue remodeling, angiogenic responses, and epithelial–mesenchymal dynamics. Dysregulated integrin signaling and altered β5-dependent adhesion programs have been implicated in models of fibrosis, tumor progression, and inflammatory microenvironment remodeling, supporting its utility in mechanistic studies of adhesion-driven phenotypes.

Integrin β5/ITGB5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Itgb5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Itgb5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Itgb5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin β5/ITGB5 protein expression.

This CRISPR knockout system enables efficient generation of Itgb5-deficient cell models for investigation of Integrin β5/ITGB5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Itgb5 exon(s) critical for Integrin β5/ITGB5 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Itgb5 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Integrin β5/ITGB5 CRISPR/Cas9 KO Plasmid (m) and Integrin β5/ITGB5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Itgb5 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Integrin β5/ITGB5 HDR Plasmid (m) and Integrin β5/ITGB5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Itgb5 homology arms to support homology-directed repair at defined Itgb5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.