Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (h): sc-401336
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α4/ITGA4/CD49d CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Integrin α4/ITGA4/CD49d genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α4/ITGA4/CD49d Antibody (C-2): sc-365569
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (h) | sc-401336 | 20 µg | $397.00 |
Overview
ITGA4 encodes integrin α4 (CD49d), an adhesion receptor that heterodimerizes with β1 (VLA-4) or β7 to mediate cell–cell and cell–matrix interactions with ligands such as VCAM-1 and fibronectin. Through integrin outside-in signaling, ITGA4 influences cytoskeletal remodeling, focal adhesion dynamics, and downstream pathways including FAK/Src, PI3K–AKT, and MAPK that govern migration, survival, and activation states. Integrin α4 is a key regulator of leukocyte trafficking, immune synapse organization, and tissue homing, linking it to inflammatory circuitry and tumor–stroma interactions. Dysregulated ITGA4 activity or expression is frequently studied in contexts of chronic inflammation, immune cell infiltration, and hematologic malignancy cell adhesion within supportive niches.
Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ITGA4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ITGA4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ITGA4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin α4/ITGA4/CD49d protein expression.
This CRISPR knockout system enables efficient generation of ITGA4-deficient cell models for investigation of Integrin α4/ITGA4/CD49d signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ITGA4 exon(s) critical for Integrin α4/ITGA4/CD49d function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ITGA4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (h) and Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ITGA4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Integrin α4/ITGA4/CD49d HDR Plasmid (h) and Integrin α4/ITGA4/CD49d HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ITGA4 homology arms to support homology-directed repair at defined ITGA4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.