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Indoxyl β-D-glucoside is a substrate used in analytical biochemistry for the detection and quantification of β-glucosidase activity. This enzyme activity is of particular interest in the study of carbohydrate metabolism and the breakdown of glucosides in both plants and microbes. When β-glucosidase acts upon Indoxyl β-D-glucoside, it releases indoxyl, which spontaneously dimerizes in the presence of oxygen to produce indigo, a blue dye. This colorimetric reaction is exploited in various assays to monitor the presence and concentration of β-glucosidase in biological samples. Moreover, Indoxyl β-D-glucoside is employed in histochemical staining techniques that enable the visualization of β-glucosidase activity in tissue sections, thereby providing insights into the spatial distribution and localization of this enzyme within different biological applications. The compound is also useful in the study of microbial ecology, as β-glucosidase activity is an important indicator of soil microorganism activity and overall soil health.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Indoxyl β-D-glucoside, 100 mg | sc-221759 | 100 mg | $120.00 | |||
Indoxyl β-D-glucoside, 500 mg | sc-221759A | 500 mg | $419.00 |