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Indoxyl β-D-glucoside (CAS 487-60-5)

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Alternate Names:
3-(β-D-Glucosido)indole, Plant indican
CAS Number:
487-60-5
Molecular Weight:
295.29
Molecular Formula:
C14H17NO6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Indoxyl β-D-glucoside is a substrate used in analytical biochemistry for the detection and quantification of β-glucosidase activity. This enzyme activity is of particular interest in the study of carbohydrate metabolism and the breakdown of glucosides in both plants and microbes. When β-glucosidase acts upon Indoxyl β-D-glucoside, it releases indoxyl, which spontaneously dimerizes in the presence of oxygen to produce indigo, a blue dye. This colorimetric reaction is exploited in various assays to monitor the presence and concentration of β-glucosidase in biological samples. Moreover, Indoxyl β-D-glucoside is employed in histochemical staining techniques that enable the visualization of β-glucosidase activity in tissue sections, thereby providing insights into the spatial distribution and localization of this enzyme within different biological applications. The compound is also useful in the study of microbial ecology, as β-glucosidase activity is an important indicator of soil microorganism activity and overall soil health.


Indoxyl β-D-glucoside (CAS 487-60-5) References

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  2. A new HPLC-ELSD method to quantify indican in Polygonum tinctorium L. and to evaluate beta-glucosidase hydrolysis of indican for indigo production.  |  Angelini, LG., et al. 2003. Biotechnol Prog. 19: 1792-7. PMID: 14656158
  3. Environmental factors affecting productivity, indican content, and indigo yield in Polygonum tinctorium Ait., a subtropical crop grown under temperate conditions.  |  Angelini, LG., et al. 2004. J Agric Food Chem. 52: 7541-7. PMID: 15675801
  4. Indigogenic substrates for detection and localization of enzymes.  |  Kiernan, JA. 2007. Biotech Histochem. 82: 73-103. PMID: 17577701
  5. Indoxyl sulfate, a uremic toxin, is biotransformed from indoxyl-beta-D-glucoside (indican) in rats.  |  Hou, YC., et al. 2008. Toxicon. 52: 440-4. PMID: 18619995
  6. Incidence of Vibrio vulnificus in estuarine waters of the south Texas Coastal Bend region.  |  Ramirez, GD., et al. 2009. J Appl Microbiol. 107: 2047-53. PMID: 19566724
  7. A modified m-CP medium for enumerating Clostridium perfringens from water samples.  |  Armon, R. and Payment, P. 1988. Can J Microbiol. 34: 78-9. PMID: 2897874
  8. Transporter-mediated interaction of indican and methotrexate in rats.  |  Lin, SP., et al. 2018. J Food Drug Anal. 26: S133-S140. PMID: 29703382
  9. Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium.  |  Inoue, S., et al. 2018. Plant Physiol Biochem. 132: 138-144. PMID: 30189417
  10. Detection of candidate proteins in the indican biosynthetic pathway of Persicaria tinctoria (Polygonum tinctorium) using protein-protein interactions and transcriptome analyses.  |  Inoue, S., et al. 2020. Phytochemistry. 179: 112507. PMID: 32931962
  11. An indigo-producing plant, Polygonum tinctorium, possesses a flavin-containing monooxygenase capable of oxidizing indole.  |  Inoue, S., et al. 2021. Biochem Biophys Res Commun. 534: 199-205. PMID: 33303189
  12. Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplasts.  |  Mellor, SB., et al. 2022. Front Plant Sci. 13: 1049177. PMID: 36743583
  13. Enumeration of Enterococcus sp. using a modified mE method.  |  Rhodes, MW. and Kator, H. 1997. J Appl Microbiol. 83: 120-6. PMID: 9246778
  14. beta-Glucosidase in the indigo plant: intracellular localization and tissue specific expression in leaves.  |  Minami, Y., et al. 1997. Plant Cell Physiol. 38: 1069-74. PMID: 9360324
  15. A rapid, specific membrane filtration procedure for enumeration of enterococci in recreational water.  |  Messer, JW. and Dufour, AP. 1998. Appl Environ Microbiol. 64: 678-80. PMID: 9464407

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Indoxyl β-D-glucoside, 100 mg

sc-221759
100 mg
$120.00

Indoxyl β-D-glucoside, 500 mg

sc-221759A
500 mg
$419.00