Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-6Rα Double Nickase Plasmid (h): sc-400582-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-6Rα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-6Rα Double Nickase Plasmid (h) and IL-6Rα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL6R. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-6Rα Antibody (H-7): sc-373708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-6Rα Double Nickase Plasmid (h) | sc-400582-NIC | 20 µg | $410.00 | |||
IL-6Rα Double Nickase Plasmid (h2) | sc-400582-NIC-2 | 20 µg | $410.00 |
Interleukin-6 receptor alpha (IL-6Rα), encoded by IL6R, is the ligand-binding subunit of the IL-6 receptor complex that initiates signaling upon IL-6 engagement and association with the GP130 co-receptor. This receptor axis activates JAK/STAT3 as well as MAPK and PI3K/AKT pathways, coordinating acute-phase responses, leukocyte differentiation, and broader inflammatory gene programs. IL6R also contributes to IL-6 trans-signaling through soluble IL-6R, extending responsiveness to cells that primarily express GP130. Dysregulated IL6R signaling has been implicated in chronic inflammation and immune-mediated pathologies and is frequently studied in contexts such as autoimmune disease biology, cancer-associated inflammation, and cardiovascular inflammatory processes.
IL-6Rα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL6R locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL6R. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL6R function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL6R-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.