IL-27Rα CRISPR/Cas9 KO Plasmid (m): sc-424540
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-27Rα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-27Rα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-27Rα CRISPR/Cas9 KO Plasmid (m) | sc-424540 | 20 µg | $397.00 |
Overview
Il27ra encodes the mouse interleukin-27 receptor alpha chain (IL-27Rα, also known as WSX-1), a cytokine receptor subunit that pairs with gp130 to form a functional IL-27 receptor complex. IL-27Rα signaling engages JAK/STAT pathways, prominently STAT1 and STAT3, to shape T cell differentiation, modulate Th1- and Th17-associated inflammatory programs, and influence cytokine production in multiple immune cell types. Through these processes, IL-27Rα contributes to regulation of immune homeostasis, antigen-driven responses, and feedback control of inflammation. Dysregulated IL-27/IL-27Rα signaling has been implicated in models of autoimmune pathology, chronic inflammation, and infection-driven immune dysfunction, supporting its use as a mechanistic node in immunology research.
IL-27Rα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il27ra gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il27ra together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il27ra open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-27Rα protein expression.
This CRISPR knockout system enables efficient generation of Il27ra-deficient cell models for investigation of IL-27Rα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il27ra exon(s) critical for IL-27Rα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il27ra genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-27Rα CRISPR/Cas9 KO Plasmid (m) and IL-27Rα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il27ra locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-27Rα HDR Plasmid (m) and IL-27Rα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il27ra homology arms to support homology-directed repair at defined Il27ra target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.