Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-1RI Double Nickase Plasmid (m): sc-421098-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1RI Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-1RI Double Nickase Plasmid (m) and IL-1RI Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Il1r1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1RI Antibody (H-8): sc-393998
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1RI Double Nickase Plasmid (m) | sc-421098-NIC | 20 µg | $410.00 | |||
IL-1RI Double Nickase Plasmid (m2) | sc-421098-NIC-2 | 20 µg | $410.00 |
Il1r1 encodes mouse IL-1RI, the primary signaling receptor for interleukin-1α and interleukin-1β that initiates innate immune activation at the cell surface. Ligand engagement recruits IL-1 receptor accessory protein and MyD88 to form a signaling complex that drives IRAK–TRAF6 cascades, culminating in NF-κB and MAPK activation and broad induction of inflammatory gene programs. Through these pathways, IL-1RI regulates cytokine and chemokine production, leukocyte recruitment, fever responses, and tissue remodeling across many cell types. Dysregulated IL-1RI signaling is implicated in models of autoinflammation, arthritis-like joint pathology, neuroinflammation, and metabolic inflammation, making Il1r1 a key node for mechanistic immunology research.
IL-1RI Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Il1r1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Il1r1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Il1r1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Il1r1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.