Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-15Rα CRISPR/Cas9 KO Plasmid (m): sc-421090
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-15Rα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-15Rα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-15Rα Antibody (G-3): sc-374023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-15Rα CRISPR/Cas9 KO Plasmid (m) | sc-421090 | 20 µg | $397.00 |
Overview
Il15ra encodes the mouse interleukin-15 receptor alpha (IL-15Rα), a high-affinity binding component that presents IL-15 in trans to IL-2/15Rβ and the common γ chain on neighboring lymphocytes. This receptor complex couples cytokine sensing to JAK/STAT signaling and supports downstream programs that regulate proliferation, survival, and differentiation of NK cells and memory-phenotype CD8+ T cells. IL-15Rα also contributes to immune homeostasis by shaping inflammatory responses and leukocyte trafficking, with implications for models of autoimmunity, chronic inflammation, infection, and tumor immunology. Genetic perturbation of Il15ra is therefore useful for dissecting cytokine-dependent crosstalk between antigen-presenting cells and responding lymphocyte subsets.
IL-15Rα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il15ra gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il15ra together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il15ra open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-15Rα protein expression.
This CRISPR knockout system enables efficient generation of Il15ra-deficient cell models for investigation of IL-15Rα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il15ra exon(s) critical for IL-15Rα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il15ra genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-15Rα CRISPR/Cas9 KO Plasmid (m) and IL-15Rα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il15ra locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-15Rα HDR Plasmid (m) and IL-15Rα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il15ra homology arms to support homology-directed repair at defined Il15ra target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.