IL-11Rα CRISPR/Cas9 KO Plasmid (h): sc-402503
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-11Rα CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-11Rα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-11Rα Antibody (4D12): sc-130920
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-11Rα CRISPR/Cas9 KO Plasmid (h) | sc-402503 | 20 µg | $397.00 |
Overview
Human IL11RA encodes the interleukin-11 receptor alpha (IL-11Rα), a ligand-binding subunit that pairs with the common signal transducer GP130 to initiate IL-11–dependent signaling. Receptor engagement activates downstream JAK/STAT, MAPK/ERK, and PI3K/AKT pathways, influencing transcriptional programs that regulate cellular differentiation, survival, and tissue remodeling. IL11RA activity is implicated in stromal–epithelial communication and regulation of inflammatory and fibrotic responses across multiple tissue contexts. Dysregulated IL-11Rα signaling has been associated with pathological remodeling phenotypes and oncogenic signaling networks, making it relevant for mechanistic studies of fibrosis- and inflammation-linked disease biology.
IL-11Rα CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IL11RA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IL11RA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IL11RA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-11Rα protein expression.
This CRISPR knockout system enables efficient generation of IL11RA-deficient cell models for investigation of IL-11Rα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IL11RA exon(s) critical for IL-11Rα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IL11RA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-11Rα CRISPR/Cas9 KO Plasmid (h) and IL-11Rα CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IL11RA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-11Rα HDR Plasmid (h) and IL-11Rα HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IL11RA homology arms to support homology-directed repair at defined IL11RA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.