Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IGFBP5 Double Nickase Plasmid (m): sc-421066-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IGFBP5 Double Nickase Plasmid (m) and IGFBP5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Igfbp5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGFBP5 Antibody (D-6): sc-515116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP5 Double Nickase Plasmid (m) | sc-421066-NIC | 20 µg | $410.00 | |||
IGFBP5 Double Nickase Plasmid (m2) | sc-421066-NIC-2 | 20 µg | $410.00 |
Mouse Igfbp5 encodes insulin-like growth factor binding protein 5 (IGFBP5), a secreted and extracellular matrix–associated regulator that modulates IGF-I/IGF-II bioavailability and signaling through the IGF1R–PI3K/AKT and MAPK pathways. Beyond IGF sequestration, IGFBP5 can influence cell adhesion, migration, and survival via interactions with ECM components and context-dependent IGF-independent activities. Igfbp5 expression is linked to tissue remodeling processes including myogenesis, osteogenesis, and fibroblast activation, making it relevant to studies of fibrosis, wound repair, and skeletal development. Dysregulated IGFBP5 has been associated with altered proliferative and differentiation programs in tumor microenvironments and stromal remodeling, supporting its use as a mechanistic node in growth factor signaling research.
IGFBP5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Igfbp5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Igfbp5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Igfbp5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Igfbp5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.