Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFIT3 Double Nickase Plasmid (h): sc-403557-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFIT3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFIT3 Double Nickase Plasmid (h) and IFIT3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFIT3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IFIT3 Antibody (B-7): sc-393512
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFIT3 Double Nickase Plasmid (h) | sc-403557-NIC | 20 µg | $410.00 | |||
IFIT3 Double Nickase Plasmid (h2) | sc-403557-NIC-2 | 20 µg | $410.00 |
Interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) is an interferon-stimulated gene product that participates in innate antiviral defense downstream of type I/III interferon and pattern-recognition receptor signaling. IFIT3 forms complexes with other IFIT family members and modulates RNA sensing, translation control, and amplification of JAK–STAT–driven interferon responses, influencing expression of broader antiviral effector programs. Through these activities, IFIT3 contributes to cellular restriction of viral replication and shapes inflammatory signaling networks such as NF-κB and IRF-dependent transcription. Dysregulated IFIT3 expression has been associated with altered antiviral responsiveness and inflammatory states, making it a useful target for mechanistic studies in infection biology, immunology, and cancer-related interferon signaling.
IFIT3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFIT3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFIT3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFIT3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFIT3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.