Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFIT2 Double Nickase Plasmid (h): sc-403830-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFIT2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFIT2 Double Nickase Plasmid (h) and IFIT2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFIT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IFIT2 Antibody (F-12): sc-390724
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFIT2 Double Nickase Plasmid (h) | sc-403830-NIC | 20 µg | $410.00 | |||
IFIT2 Double Nickase Plasmid (h2) | sc-403830-NIC-2 | 20 µg | $410.00 |
Interferon induced protein with tetratricopeptide repeats 2 (IFIT2) is an interferon-stimulated gene encoding a cytosolic antiviral effector that binds RNA species and modulates translation and RNA metabolism during innate immune responses. IFIT2 is induced downstream of type I interferon signaling via JAK–STAT and ISGF3-driven transcription, integrating into broader antiviral programs alongside other IFIT family members. Through interactions with viral and host RNAs and regulation of stress and apoptotic pathways, IFIT2 contributes to restriction of viral replication and shaping inflammatory outputs. Dysregulated IFIT2 expression has been associated with altered interferon signatures observed in infection, cancer biology, and immune-mediated disorders, making it a useful node for dissecting interferon pathway regulation.
IFIT2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFIT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFIT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFIT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFIT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.