IER2 CRISPR/Cas9 KO Plasmid (h): sc-407175
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IER2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IER2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IER2 CRISPR/Cas9 KO Plasmid (h) | sc-407175 | 20 µg | $397.00 |
Overview
Immediate early response 2 (IER2) is a rapidly induced, stimulus-responsive gene that integrates extracellular cues from growth factors, stress signals, and mitogenic inputs into short-lived transcriptional programs. IER2 activity has been linked to regulation of cell migration, cytoskeletal remodeling, and context-dependent control of proliferation through MAPK/ERK-associated immediate early signaling and downstream gene expression changes. As an immediate early effector, IER2 helps shape early transcriptional network rewiring that influences adhesion dynamics and invasive behavior in multiple cellular models. Altered IER2 expression has been reported across disease-relevant settings, supporting its use as a mechanistic node for studying signaling-to-transcription coupling in cancer and inflammation-related biology.
IER2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IER2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IER2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IER2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IER2 protein expression.
This CRISPR knockout system enables efficient generation of IER2-deficient cell models for investigation of IER2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IER2 exon(s) critical for IER2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IER2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IER2 CRISPR/Cas9 KO Plasmid (h) and IER2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IER2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IER2 HDR Plasmid (h) and IER2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IER2 homology arms to support homology-directed repair at defined IER2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.