IDH1 Double Nickase Plasmid (h): sc-401159-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IDH1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IDH1 Double Nickase Plasmid (h) and IDH1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IDH1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IDH1 Double Nickase Plasmid (h) | sc-401159-NIC | 20 µg | $410.00 | |||
IDH1 Double Nickase Plasmid (h2) | sc-401159-NIC-2 | 20 µg | $410.00 |
Isocitrate dehydrogenase 1 (IDH1) is a cytosolic/peroxisomal NADP⁺-dependent enzyme that catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate, generating NADPH to support redox homeostasis and biosynthetic metabolism. Through control of α-ketoglutarate availability, IDH1 influences lipid synthesis, cellular antioxidant capacity, and the activity of α-ketoglutarate–dependent dioxygenases involved in epigenetic regulation. Altered IDH1 function is linked to metabolic rewiring and disrupted chromatin states, including well-characterized neomorphic variants that produce 2-hydroxyglutarate and perturb DNA/histone demethylation. These properties make IDH1 a central node for studying metabolic–epigenetic coupling, oxidative stress responses, and context-specific vulnerabilities in human cells.
IDH1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IDH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IDH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IDH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IDH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.