Anti-I2PP2A Anticuerpo F-9 es un monoclonal de ratón IgG2a κ I2PP2A Anticuerpo, ver las 10 publicaciones, proporcionado como 200 µg/ml
producido contra los amino ácidos 1-120 de I2PP2A de origen human
I2PP2A Anticuerpo (F-9) es recomendado para detectar I2PP2A de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
I2PP2A Anticuerpo (F-9) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de I2PP2A (F-9): sc-133138.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for I2PP2A Antibody (F-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with I2PP2A Antibody (F-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I2PP2A Antibody (F-9) is a high quality monoclonal I2PP2A antibody (also designated HLA-DR associated protein II antibody, PHAPII antibody, Phosphatase 2A inhibitor IPP2A2 antibody, Template-Activating Factor-I antibody, TAF-IBETA antibody, Inhibitor of granzyme A-activated DNase antibody, IGAAD antibody, or nucleosome acetylation inhibitory complex component antibody) suitable for the detection of the I2PP2A protein of mouse, rat and human origin. I2PP2A Antibody (F-9) is available as both the non-conjugated anti-I2PP2A antibody form, as well as multiple conjugated forms of anti-I2PP2A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Protein phosphatase 2A PP2A is a major mammalian protein serine/threonine phosphatase that regulates diverse cellular processes. Inhibitor 1 of PP2A (I1PP2A) and inhibitor 2 of PP2A (I2PP2A), which share large sequence similarity, are heat-stable protein inhibitors of the cellular phosphatase activity of PP2A. I1PP2A and I2PP2A were initially characterized as putative HLA class II associated proteins Phap I and Phap II. These inhibitor proteins act noncompetitively to selectively inhibit PP2A, but do not affect the phosphatase activity of the related proteins PP1, PP2B and PP2C. The I1PP2A protein is localized to both the cytoplasm and the nucleus. In contrast, I2PP2A is located predominantly in the nucleus and is highly expressed in Wilms′ tumor cells. Transient expression of I2PP2A in HEK-293 cells leads to an increase in the DNA binding activity of the proto-oncogene c-Jun.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Secuencia completa de la proteina I2PP2A con el immunogeno subrayado:
Thanks for your question.I2PP2A Antibody (F-9): sc-133138 is raised against amino acids 1-120 of I2PP2A of human origin.Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer if this primary antibody can reactive with your target protein . Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Respondida por: Technical Support 9
Fecha de publicación: 2020-07-10
Can this antibody be used to detect I2PP2A of bovine origin?
Preguntado por: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Rated 4 de
good productWe got clean band for WB experiment with some background.
Fecha de publicación: 2018-09-11
Rated 3 de
Nice antibody for WB!This antibody works fine to detect I2PP2A in WB in human samples. In my hands it did not work in IF.
Fecha de publicación: 2018-04-03
Rated 5 de
Specific band in Western BlotI got the antibody as a free sample to test for Western Blot. It arrived very quickly and it works really nice for Western Blot. I used it in a 1:500 dilution (recommended was to start with a 1:100 dilution).
Fecha de publicación: 2018-02-02
Rated 5 de
Excellent antibodyI received this antibody as a sample and used it to probe rat astrocyte lysates. I worked just as predicted. Blots were very clean when I used the IgG kappa chain secondary. See image.
Fecha de publicación: 2017-10-05
Rated 5 de
western blotI used this antibody for western blot in a dilution of 1:2000 with great results. The signal was very strong, however. No nonspecific bands were detected.
Fecha de publicación: 2017-08-19
Rated 5 de
Robust signal for WBI'm glad to use it and get good results with it in WB.
Fecha de publicación: 2017-05-31
Rated 5 de
Positive resultPositive WB data in DU 145 whole cell lysate,strong band seen.
Fecha de publicación: 2017-05-25
Rated 5 de
worked niceIt worked nice when I used for WB of mouse liver tissue,I use the dilution 1:500.
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