HYPB CRISPR/Cas9 KO Plasmid (h): sc-402789
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HYPB CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HYPB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HYPB CRISPR/Cas9 KO Plasmid (h) | sc-402789 | 20 µg | $397.00 |
Overview
SETD2, also known as HYPB, encodes the principal histone methyltransferase responsible for trimethylation of histone H3 lysine 36 (H3K36me3) across actively transcribed gene bodies. This modification coordinates transcriptional elongation with co-transcriptional RNA processing, chromatin organization, and recruitment of DNA repair machinery, linking SETD2 activity to genome stability maintenance. SETD2-dependent H3K36me3 also helps specify repair pathway choice during homologous recombination and supports proper replication fork dynamics. Altered SETD2 function and disrupted H3K36 methylation patterns are frequently studied in the context of oncogenic epigenetic deregulation, transcriptional stress, and DNA damage response defects.
HYPB CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SETD2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SETD2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SETD2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HYPB protein expression.
This CRISPR knockout system enables efficient generation of SETD2-deficient cell models for investigation of HYPB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SETD2 exon(s) critical for HYPB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SETD2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HYPB CRISPR/Cas9 KO Plasmid (h) and HYPB CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SETD2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HYPB HDR Plasmid (h) and HYPB HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SETD2 homology arms to support homology-directed repair at defined SETD2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.