Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HXK I CRISPR/Cas9 KO Plasmid (h): sc-401753
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HXK I CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HXK I genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HXK I Antibody (G-1): sc-46695
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HXK I CRISPR/Cas9 KO Plasmid (h) | sc-401753 | 20 µg | $397.00 |
Overview
Human HK1 encodes hexokinase I (HXK I), a mitochondrial-associated enzyme that catalyzes the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, committing glucose to glycolysis and related biosynthetic routes. By controlling the first rate-limiting step of glucose utilization, HXK I influences cellular energy balance, redox homeostasis through coupling to the pentose phosphate pathway, and metabolite flux into glycogen and nucleotide synthesis. HK1 activity is tightly integrated with mitochondrial function and apoptosis-associated signaling via interactions at the outer mitochondrial membrane. Altered HK1 expression or regulation has been linked to dysregulated glucose metabolism in cancer and to neurodevelopmental and hemolytic phenotypes, supporting its relevance in metabolic and mitochondrial biology research.
HXK I CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HK1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HK1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HK1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HXK I protein expression.
This CRISPR knockout system enables efficient generation of HK1-deficient cell models for investigation of HXK I signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HK1 exon(s) critical for HXK I function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HK1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HXK I CRISPR/Cas9 KO Plasmid (h) and HXK I CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HK1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HXK I HDR Plasmid (h) and HXK I HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HK1 homology arms to support homology-directed repair at defined HK1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.